RBM8A antibody (full length)

Catalog No. ABIN7878929

This Mouse Monoclonal antibody specifically detects RBM8A in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for RBM8A antibody (full length) (ABIN7878929)

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBM8A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-RBM8A-1B4

Product Details anti-RBM8A Antibody

Binding Specificity: full length

Purpose: RBM8A/Y14 Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human RBM8A protein was used as the immunogen for the RBM8A/Y14 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the RBM8A/Y14 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the RBM8A/Y14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for RBM8A

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Alternative Name: RBM8A/Y14

Background: The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

UniProt: Q9Y5S9