ube3a antibody (full length)

Catalog No. ABIN7879289

This Mouse Monoclonal antibody specifically detects ube3a in IF and FACS. It exhibits reactivity toward Human.

Quick Overview for ube3a antibody (full length) (ABIN7879289)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: PCRP-UBE3A-1A2

Product Details anti-ube3a Antibody

Binding Specificity: full length

Purpose: Ubiquitin-protein ligase E3A Antibody / UBE3A (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human UBE3A protein was used as the immunogen for the Ubiquitin-protein ligase E3A antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the Ubiquitin-protein ligase E3A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the Ubiquitin-protein ligase E3A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: Ubiquitin-protein ligase E3A

Background: E6-associating protein (E6-AP), also designated ubiquitin protein ligase E3A (UBE3A), is a component of the ubiquitin-mediated proteolytic pathway that selectively targets proteins for degradation by the 26S Proteasome. Ubiquitin(Ub) is directly conjugated to protein substrates by the transfer of Ub from anE2 ubiquitin conjugating enzyme to the target protein. This conjugation is facilitated by the enzymatic activity of E3 ubiquitin ligase family members such asE6-AP. Several substrates of E6-AP have been identified and include the tumor suppressor protein p53 and the mammalian homolog of Rad23, HHR23A.Previous studies have indicated that E6-AP associates with the human papilloma virus E6 oncogene, which forms a complex with p53 and there by potentiates E6-AP mediated ubiquitination of p53. Genetic mutations that impair E6-AP activity result in the accumulation of p53 in the cytoplasm, and in many instances, these mutations are associated with the development of the rare neurodevelopmental disorder Angelman syndrome (AS), which is characterized by severe motor dysfunction and mental retardation.

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway