Vinculin antibody (full length)

Catalog No. ABIN7879318

The Mouse Monoclonal anti-Vinculin antibody (Clone VCL-3617) (ABIN7879318) specifically detects Vinculin in IF, IHC (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for Vinculin antibody (full length) (ABIN7879318)

Target: Vinculin (VCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: VCL-3617

Product Details anti-Vinculin Antibody

Binding Specificity: full length

Purpose: Vinculin Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human Vinculin protein was used as the immunogen for the Vinculin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Vinculin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Vinculin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: This antibody recognizes both Vinculin (125 kDa) and meta-Vinculin (135 kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2