Histone H2A antibody (H2AS139ph)

Catalog No. ABIN7879416

The Mouse Monoclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human and Mouse. It has been validated for WB and IF.

Quick Overview for Histone H2A antibody (H2AS139ph) (ABIN7879416)

Target: Histone H2A

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Histone H2A Antibody

Binding Specificity: H2AS139ph

Purpose: Phospho-Histone H2AX Antibody (pS139)

Purification: Antigen affinity purified

Immunogen: The amino acid sequence surrounding phosphorylated serine 139 was used as the immunogen for the Phospho-Histone H2AX antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Titration of the Phospho-Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Phospho-Histone H2AX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Histone H2A

Target: Histone H2A

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

UniProt: P16104