KIF7 antibody (Middle Region)

Catalog No. ABIN7879526

This Rabbit Polyclonal antibody specifically detects KIF7 in WB. It exhibits reactivity toward Human.

Quick Overview for KIF7 antibody (Middle Region) (ABIN7879526)

Target: KIF7 (Kinesin Family Member 7 (KIF7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KIF7 Antibody

Binding Specificity: Middle Region

Purpose: KIF7 Antibody / Kinesin-like protein KIF7

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human KIF7 was used as the immunogen for the KIF7 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the KIF7 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the KIF7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for KIF7

Target: KIF7 (Kinesin Family Member 7 (KIF7))

Alternative Name: KIF7

Background: KIF7 antibody detects Kinesin-like protein KIF7, a microtubule-associated motor protein that plays a crucial role in Hedgehog signaling and ciliary function. KIF7 is a member of the kinesin superfamily and acts as a regulator of microtubule dynamics rather than as a transport motor. The KIF7 antibody is commonly used in developmental biology and cilia research to explore signal transduction, cytoskeletal regulation, and morphogenesis.

KIF7 is encoded by the KIF7 gene located on human chromosome 15q26.1. The protein is approximately 151 kilodaltons and contains an N-terminal motor domain, a coiled-coil stalk, and a C-terminal tail region required for signaling complex assembly. KIF7 localizes to the primary cilium and functions as a scaffold controlling the balance between active and repressive forms of the transcription factor GLI in response to Hedgehog pathway activation.

The KIF7 antibody detects a 134 kilodalton protein by western blot and reveals distinct ciliary tip staining by immunofluorescence. KIF7 regulates microtubule stability and controls ciliary length, essential for proper Hedgehog signal transduction. It restrains GLI2 and GLI3 activity by preventing excessive microtubule polymerization at the ciliary tip and recruiting pathway regulators such as SUFU.

Loss-of-function mutations in KIF7 cause ciliopathies, including Joubert syndrome and hydrolethalus syndrome, characterized by brain malformations and defective patterning during embryogenesis. Aberrant KIF7 signaling disrupts tissue polarity and organ development, while partial reduction contributes to tumorigenesis by allowing uncontrolled Hedgehog pathway activation.

KIF7 is an essential modulator of ciliary signaling and structural organization, integrating microtubule mechanics with developmental cues. NSJ Bioreagents provides a validated KIF7 antibody optimized for its applications, enabling detailed investigation of Hedgehog pathway regulation, neuronal patterning, and ciliary function.

UniProt: Q2M1P5

Pathways: Hedgehog Signaling