CREB3L2 antibody (N-Term)

Catalog No. ABIN7879572

The Rabbit Polyclonal anti-CREB3L2 antibody is suitable to detect CREB3L2 in samples from Human. It has been validated for WB, IHC, IF, ICC and FACS.

Quick Overview for CREB3L2 antibody (N-Term) (ABIN7879572)

Target: CREB3L2 (CAMP Responsive Element Binding Protein 3-Like 2 (CREB3L2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB3L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-CREB3L2 Antibody

Binding Specificity: N-Term

Purpose: CREB3L2 Antibody / Cyclic AMP-responsive element-binding protein 3-like protein 2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CREB3L2 was used as the immunogen for the CREB3L2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CREB3L2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CREB3L2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CREB3L2

Target: CREB3L2 (CAMP Responsive Element Binding Protein 3-Like 2 (CREB3L2))

Alternative Name: CREB3L2

Background: CREB3L2 antibody detects Cyclic AMP-responsive element-binding protein 3-like protein 2, a transcription factor that regulates endoplasmic reticulum (ER) stress response and secretory pathway gene expression. The UniProt recommended name is Cyclic AMP-responsive element-binding protein 3-like protein 2 (CREB3L2). This ER membrane-bound transcription factor modulates secretion, collagen synthesis, and stress adaptation through transcriptional control of Golgi and ER-resident proteins.

Functionally, CREB3L2 antibody identifies a 520-amino-acid protein anchored in the ER membrane via a C-terminal transmembrane domain. Upon ER stress or secretory stimulation, the N-terminal cytosolic fragment of CREB3L2 is cleaved and translocates to the nucleus, where it binds CRE-like sequences in target promoters to activate genes related to vesicle trafficking, extracellular matrix organization, and stress tolerance. CREB3L2 acts in concert with other bZIP transcription factors such as ATF6 and CREB3L1 to regulate protein folding and secretion.

The CREB3L2 gene is located on chromosome 7q33 and is highly expressed in secretory tissues such as cartilage, liver, and endocrine glands. It contributes to normal skeletal development and cellular adaptation to increased protein synthesis demands. CREB3L2 is also a critical determinant of collagen biosynthesis during chondrogenesis and wound repair.

Pathologically, translocations involving CREB3L2 and FUS lead to low-grade fibromyxoid sarcoma, resulting in constitutive transcriptional activation and altered differentiation. Aberrant CREB3L2 activity has also been implicated in metabolic and neurodegenerative disorders linked to ER stress. Research using CREB3L2 antibody supports studies in transcriptional control, ER stress, and tumor biology.

CREB3L2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ER-associated transcription factors. NSJ Bioreagents provides CREB3L2 antibody reagents optimized for studies in transcriptional regulation, protein secretion, and cellular stress responses.

Structurally, Cyclic AMP-responsive element-binding protein 3-like protein 2 features a basic leucine zipper (bZIP) DNA-binding domain, an ER luminal domain involved in sensing stress signals, and a transmembrane region that anchors it to the ER. This antibody aids in studying CREB3L2's role in secretory pathway regulation, collagen production, and tumorigenic gene fusion mechanisms.

UniProt: Q70SY1

Pathways: Thyroid Hormone Synthesis