Recombinant DPYSL2 antibody (pThr514)

Catalog No. ABIN7879800

The Rabbit Monoclonal anti-DPYSL2 antibody is suitable to detect DPYSL2 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Recombinant DPYSL2 antibody (pThr514) (ABIN7879800)

Target: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DPYSL2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32D10

Product Details anti-DPYSL2 Antibody

Binding Specificity: pThr514

Purpose: Phospho-CRMP2 (Thr514) Antibody / Collapsin response mediator protein 2

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-CRMP2 (T514) was used as the immunogen for the Phospho-CRMP2 (Thr514) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-CRMP2 (Thr514) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-CRMP2 (Thr514) antibody at -20oC.

Target Details for DPYSL2

Target: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Alternative Name: CRMP2

Background: Phospho-CRMP2 (Thr514) antibody detects collapsin response mediator protein 2 when phosphorylated at threonine 514. CRMP2, encoded by the DPYSL2 gene, is a cytosolic phosphoprotein that regulates axon guidance, neuronal polarity, and microtubule dynamics. Phosphorylation at Thr514, primarily mediated by GSK3 beta, reduces CRMP2 binding to tubulin and destabilizes microtubules. This modification plays critical roles in neuronal development and synaptic remodeling.

Phospho-CRMP2 (Thr514) antibody is widely applied in neuroscience research, particularly in studies of axon guidance and neurodegeneration. By detecting phosphorylated CRMP2, researchers can distinguish between active and inhibited states of the protein. Elevated phosphorylation of CRMP2 at Thr514 has been associated with Alzheimer disease and other neurodegenerative conditions where microtubule stability is compromised.

The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-CRMP2 (Thr514) antibody detects distinct phosphorylated isoforms. Immunohistochemistry highlights tissue specific phosphorylation patterns in brain regions, while immunofluorescence enables visualization of phospho CRMP2 at growth cones and synapses. These applications make the antibody valuable for developmental neuroscience and disease studies.

Phosphorylation of CRMP2 at Thr514 regulates not only axonal outgrowth but also dendritic spine morphology and synaptic plasticity. Dysregulation of this site contributes to cognitive decline and neuropsychiatric disorders. By applying Phospho-CRMP2 (Thr514) antibody, scientists can explore mechanisms linking abnormal phosphorylation to neuronal dysfunction and identify therapeutic approaches that target CRMP2 signaling.

Phospho-CRMP2 (Thr514) antibody from NSJ Bioreagents provides reliable specificity for detecting phosphorylated DPYSL2, enabling accurate monitoring of microtubule regulatory pathways in health and disease.

UniProt: Q16555

Pathways: Regulation of Cell Size