Recombinant TBC1D4 antibody (pThr642)

Catalog No. ABIN7879804

The Rabbit Monoclonal anti-TBC1D4 antibody (Clone 31T47) (ABIN7879804) specifically detects TBC1D4 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant TBC1D4 antibody (pThr642) (ABIN7879804)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31T47

Product Details anti-TBC1D4 Antibody

Binding Specificity: pThr642

Purpose: Phospho-AS160 (Thr642) Antibody / TBC1D4

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-AS160 (T642) was used as the immunogen for the Phospho-AS160 (Thr642) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-AS160 (Thr642) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-AS160 (Thr642) antibody at -20oC.

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: AS160

Background: Phospho-AS160 (Thr642) antibody detects TBC1 domain family member 4 when phosphorylated at threonine 642. This protein is encoded by the TBC1D4 gene and is commonly referred to as AS160, Akt substrate of 160 kDa, and insulin-regulated Rab GTPase-activating protein. TBC1D4 functions as a Rab GAP that regulates trafficking of GLUT4 vesicles in response to insulin signaling. Phosphorylation at Thr642 by Akt relieves its inhibitory GAP activity, allowing Rab GTPases to promote translocation of GLUT4 to the plasma membrane, a critical event in glucose uptake by adipocytes and muscle cells.

Phospho-AS160 (Thr642) antibody is widely applied in metabolism, endocrinology, and diabetes research. Detecting phosphorylation at Thr642 provides a direct readout of insulin-stimulated Akt signaling and GLUT4 trafficking. This makes the antibody valuable for studies of insulin resistance, obesity, and type 2 diabetes. In addition, TBC1D4 is expressed in other tissues, linking it to energy homeostasis beyond classical insulin-responsive cells.

Applications for Phospho-AS160 (Thr642) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylation changes in response to insulin or exercise, immunohistochemistry maps expression in metabolic tissues, and immunofluorescence highlights vesicle trafficking events. These methods allow researchers to connect molecular signaling to physiological glucose uptake.

Dysregulation of TBC1D4 phosphorylation is linked to insulin resistance and diabetes. Reduced phosphorylation impairs GLUT4 trafficking, while mutations in TBC1D4 cause severe postprandial hyperinsulinemia and muscle glycogen storage abnormalities. By applying Phospho-AS160 (Thr642) antibody, scientists can study both common and rare metabolic disorders.

Beyond metabolism, TBC1D4 regulates vesicle trafficking in immune cells, neurons, and other systems. Phosphorylation-dependent regulation highlights its integration with Akt signaling in multiple contexts. NSJ Bioreagents provides Phospho-AS160 (Thr642) antibody with validated specificity, ensuring accurate detection of this phosphorylation event in insulin and trafficking research.

UniProt: O60343