Recombinant SYK antibody (pTyr323)

Catalog No. ABIN7879832

This Rabbit Monoclonal antibody specifically detects SYK in WB, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant SYK antibody (pTyr323) (ABIN7879832)

Target: SYK (Spleen tyrosine Kinase (SYK))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 32S11

Product Details anti-SYK Antibody

Binding Specificity: pTyr323

Purpose: Phospho-Syk (Tyr323) Antibody / SYK

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-Syk (Y323) was used as the immunogen for the Phospho-Syk (Tyr323) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-Syk (Tyr323) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-Syk (Tyr323) antibody at -20oC.

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: Syk

Background: Phospho-Syk (Tyr323) antibody detects Spleen tyrosine kinase phosphorylated at tyrosine 323, encoded by the SYK gene. Spleen tyrosine kinase is a cytoplasmic non receptor tyrosine kinase that mediates signaling downstream of immunoreceptors such as the B cell receptor, Fc receptors, and integrins. Phosphorylation at tyrosine 323 is a critical modification that regulates kinase activity and adaptor interactions. Phospho-Syk (Tyr323) antibody provides a selective tool for studying immune signaling, B cell activation, and inflammatory responses.

Spleen tyrosine kinase is activated when immunoreceptors are crosslinked, leading to phosphorylation of ITAM motifs and recruitment of SYK through its tandem SH2 domains. Once engaged, SYK becomes phosphorylated at multiple sites, including tyrosine 323, which enhances its catalytic activity and binding to signaling partners. Research with Phospho-Syk (Tyr323) antibody has demonstrated that this phosphorylation event promotes propagation of signaling cascades leading to calcium flux, MAP kinase activation, and transcriptional responses. These events are fundamental for lymphocyte activation and immune defense.

Beyond lymphocytes, SYK plays roles in innate immune cells, including macrophages, mast cells, and neutrophils. Studies with Phospho-Syk (Tyr323) antibody have shown that phosphorylation at this site regulates Fc receptor signaling, phagocytosis, and cytokine release. Dysregulation of SYK phosphorylation has been linked to autoimmune disease, allergy, and chronic inflammation, making it a therapeutic target. Inhibitors of SYK are in clinical development for treatment of rheumatoid arthritis, lupus, and hematologic malignancies.

Phosphorylation at tyrosine 323 also contributes to oncogenic signaling. Research with Phospho-Syk (Tyr323) antibody has revealed aberrant activation in leukemias and lymphomas, where it drives proliferation and survival. Monitoring phosphorylation state is therefore critical for understanding pathogenesis and evaluating targeted therapies. Because SYK functions at the intersection of immunity and cancer, its phosphorylation events remain of high biomedical importance.

Phospho-Syk (Tyr323) antibody is used in western blotting, immunohistochemistry, and flow cytometry. Western blotting distinguishes phosphorylated and unphosphorylated forms, immunohistochemistry localizes activated kinase in tissue samples, and flow cytometry quantifies phosphorylation dynamics in immune cells. These approaches make Phospho-Syk (Tyr323) antibody essential for dissecting immune receptor signaling.

By supplying validated Phospho-Syk (Tyr323) antibody reagents, NSJ Bioreagents supports research into immune signaling, autoimmunity, and cancer. Detection of Spleen tyrosine kinase phosphorylation at tyrosine 323 provides a precise measure of immune activation.

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling