Recombinant BCAR1 antibody (pTyr410)

Catalog No. ABIN7879834

The Rabbit Monoclonal anti-BCAR1 antibody (Clone 32B03) (ABIN7879834) specifically detects BCAR1 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant BCAR1 antibody (pTyr410) (ABIN7879834)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32B03

Product Details anti-BCAR1 Antibody

Binding Specificity: pTyr410

Purpose: Phospho-BCAR1 (Tyr410) Antibody / Breast cancer anti-estrogen resistance protein 1

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-BCAR1 (Y410) was used as the immunogen for the Phospho-BCAR1 (Tyr410) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-BCAR1 (Tyr410) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-BCAR1 (Tyr410) antibody at -20oC.

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1

Background: Phospho-BCAR1 (Tyr410) antibody detects breast cancer anti-estrogen resistance protein 1 when phosphorylated at tyrosine 410. BCAR1, also known as p130Cas, is an adaptor protein encoded by the BCAR1 gene. It localizes to focal adhesions where it links integrins and growth factor receptors to downstream signaling pathways. Phosphorylation at Tyr410 is a critical event that promotes protein protein interactions and assembly of signaling complexes regulating adhesion, migration, and survival.

Phospho-BCAR1 (Tyr410) antibody is widely used to study integrin signaling, cytoskeletal dynamics, and cancer biology. BCAR1 phosphorylation at focal adhesions enables activation of Src family kinases and downstream effectors such as Rac and JNK. By detecting the phosphorylated form of BCAR1, this antibody provides insight into how cells sense and respond to extracellular matrix cues.

The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-BCAR1 (Tyr410) antibody detects phosphorylated isoforms distinct from non phosphorylated protein. Immunohistochemistry maps phosphorylation events in tissue samples, while immunofluorescence reveals spatial localization of activated BCAR1 at focal adhesions. These approaches allow researchers to monitor cell adhesion signaling with high precision.

BCAR1 phosphorylation has been linked to oncogenesis and therapy resistance. Elevated phospho BCAR1 levels are associated with enhanced tumor invasion and poor prognosis in breast and other cancers. By using Phospho-BCAR1 (Tyr410) antibody, scientists can evaluate how dysregulated adhesion signaling contributes to malignancy and whether Tyr410 phosphorylation serves as a biomarker of tumor progression.

Beyond oncology, BCAR1 phosphorylation plays roles in cardiovascular and immune systems. It regulates endothelial cell migration during angiogenesis and contributes to immune cell trafficking. In developmental contexts, BCAR1 activity is essential for morphogenesis and tissue patterning. The phospho specific antibody enables detailed investigation of these roles across models.

Phospho-BCAR1 (Tyr410) antibody from NSJ Bioreagents provides strong specificity for activated BCAR1, supporting studies of integrin signaling, migration, and cancer biology. Its proven reliability ensures accurate detection of phosphorylation events in multiple research applications.

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling