Recombinant AZI2 antibody

Catalog No. ABIN7879852

The Rabbit Monoclonal anti-AZI2 antibody (Clone 30A13) (ABIN7879852) specifically detects AZI2 in WB, IF and ICC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Recombinant AZI2 antibody (ABIN7879852)

Target: AZI2 (5-Azacytidine Induced 2 (AZI2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AZI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 30A13

Product Details anti-AZI2 Antibody

Purpose: AZI2 Antibody / 5-Azacytidine-induced protein 2

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human TAF3 was used as the immunogen for the AZI2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the AZI2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the AZI2 antibody at -20oC.

Target Details for AZI2

Target: AZI2 (5-Azacytidine Induced 2 (AZI2))

Alternative Name: AZI2

Background: AZI2 antibody is designed to detect 5-azacytidine-induced protein 2, also known as NAP1 or TBK1-binding protein 1. AZI2 is a cytoplasmic protein that plays a significant role in innate immunity by regulating signaling pathways that activate interferon regulatory factors (IRFs) and nuclear factor kappa B (NF-kB). Through its interactions with kinases such as TBK1 and IKKe, AZI2 contributes to the activation of interferon-stimulated genes and inflammatory mediators that defend the host against viral and bacterial infections.

AZI2 antibody has become a valuable reagent for immunology and virology research. The protein is induced in response to 5-azacytidine and is involved in noncanonical NF-kB signaling. It is known to function in concert with NAP1, facilitating the recruitment of kinases that phosphorylate IRF3 and IRF7, leading to interferon beta production. Detecting AZI2 expression and localization provides insight into how innate immune responses are initiated and regulated at the molecular level.

Applications of AZI2 antibody include western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, AZI2 antibody identifies the protein with specificity, allowing researchers to monitor expression in cells exposed to pathogens or immune modulators. In immunohistochemistry, it reveals tissue-specific distribution patterns, while immunofluorescence highlights cytoplasmic localization and colocalization with signaling proteins. These applications make AZI2 antibody versatile for dissecting immune signaling pathways in diverse research contexts.

AZI2 is particularly important in antiviral defense. Studies have demonstrated that AZI2 participates in signaling cascades activated by Toll-like receptors (TLRs) and RIG-I-like receptors (RLRs), key components of pathogen recognition. Altered AZI2 expression can impair interferon responses, contributing to viral persistence or inadequate immune control. By using AZI2 antibody, researchers can track changes in expression that correlate with immune activation and disease outcomes.

In addition to innate immunity, AZI2 has been implicated in cancer biology and inflammation. Dysregulation of NF-kB and interferon pathways is common in tumor microenvironments, where chronic inflammation promotes cancer progression. Monitoring AZI2 expression with specific antibodies supports studies aimed at understanding how immune signaling intersects with oncogenesis.

AZI2 antibody from NSJ Bioreagents provides researchers with a dependable reagent to study innate immunity, viral infection, and NF-kB signaling. Its proven specificity across assay platforms ensures accurate detection, supporting investigations into host defense and immune regulation.

UniProt: Q9H6S1