Recombinant Actin antibody

Catalog No. ABIN7879866

The Rabbit Monoclonal anti-Actin antibody is suitable to detect Actin in samples from Human. It has been validated for ELISA.

Quick Overview for Recombinant Actin antibody (ABIN7879866)

Target: Actin

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: ELISA

Clone: MSA-8949R

Product Details anti-Actin Antibody

Purpose: Actin Antibody / Muscle specific

Purification: Protein A/G affinity

Immunogen: Recombinant human muscle actin protein was used as the immunogen for the Actin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the Actin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Actin

Target: Actin

Background: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

UniProt: P62736