ATG4A antibody

Catalog No. ABIN7880031

The Mouse Monoclonal anti-ATG4A antibody is suitable to detect ATG4A in samples from Human. It has been validated for WB, IF, IHC (p) and FACS.

Quick Overview for ATG4A antibody (ABIN7880031)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG4A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 1458CT808-66-25-69

Product Details anti-ATG4A Antibody

Purpose: ATG4A Antibody

Purification: Protein G affinity

Immunogen: Recombinant human protein was used as the immunogen for the ATG4A antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the ATG4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot the ATG4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Alternative Name: ATG4A

Background: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

UniProt: Q8WYN0

Pathways: Autophagy