Caldesmon antibody

Catalog No. ABIN7880139

The Mouse Monoclonal anti-Caldesmon antibody is suitable to detect Caldesmon in samples from Human. It has been validated for IHC (p).

Quick Overview for Caldesmon antibody (ABIN7880139)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM168

Product Details anti-Caldesmon Antibody

Purpose: Caldesmon Antibody / CALD1 / CAD (HMW)

Purification: Protein G affinity chromatography

Immunogen: Crude human uterus extract was used as the immunogen for the Caldesmon antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Caldesmon antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Caldesmon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: Caldesmon

Background: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction