CD45RB antibody

Catalog No. ABIN7880313

The Mouse Monoclonal anti-CD45RB antibody is suitable to detect CD45RB in samples from Human. It has been validated for IHC (p).

Quick Overview for CD45RB antibody (ABIN7880313)

Target: CD45RB

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RB antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM569

Product Details anti-CD45RB Antibody

Purpose: Anti-CD45RB Antibody

Purification: Protein G affinity chromatography

Immunogen: Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this anti-CD45RB antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-CD45RB antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-CD45RB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB

Background: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

UniProt: P08575