Recombinant Nesprin3 antibody

Catalog No. ABIN7880434

The Rabbit Monoclonal anti-Nesprin3 antibody (Clone 30S39) (ABIN7880434) specifically detects Nesprin3 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant Nesprin3 antibody (ABIN7880434)

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nesprin3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 30S39

Product Details anti-Nesprin3 Antibody

Purpose: Nesprin 3 Antibody / SYNE3

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Nesprin3 was used as the immunogen for the Nesprin 3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Nesprin 3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Nesprin 3 antibody at -20oC.

Target Details for Nesprin3

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Alternative Name: Nesprin 3

Background: Nesprin 3 antibody detects Spectrin repeat containing nuclear envelope protein 3, encoded by the SYNE3 gene. This protein, commonly known as Nesprin 3, is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex that spans the nuclear envelope. Unlike other Nesprin family members that link directly to actin, Nesprin 3 connects to intermediate filaments through interactions with plectin. Nesprin 3 antibody provides researchers with a key reagent to study nuclear positioning, mechanotransduction, and cytoskeletal organization.

The LINC complex plays a central role in transmitting mechanical forces between the cytoskeleton and the nucleus. By tethering the nuclear envelope to intermediate filament networks, Nesprin 3 contributes to nuclear anchorage during migration, differentiation, and mitosis. Research using Nesprin 3 antibody has shown that loss of this protein disrupts nuclear shape and mechanosensing, leading to altered transcriptional responses. These findings emphasize the importance of Nesprin 3 in structural integrity and gene regulation.

Dysfunction of Nesprin proteins, including Nesprin 3, has been linked to muscular dystrophies, cardiomyopathies, and neurological disorders. While most studies have focused on Nesprin 1 and Nesprin 2, evidence suggests that Nesprin 3 contributes to nuclear envelope stability and tissue homeostasis. Studies using Nesprin 3 antibody have associated its altered expression with cancer progression, where changes in nuclear deformability affect invasion and metastasis. These roles highlight the significance of SYNE3 in both health and disease.

Nesprin 3 antibody is widely applied in western blotting, immunofluorescence, and immunohistochemistry. Western blotting demonstrates isoform expression, immunofluorescence shows its localization at the nuclear envelope, and immunohistochemistry reveals its distribution in tissues such as muscle, epithelium, and nervous system. Functional studies have employed Nesprin 3 antibody in knockdown and rescue experiments, linking its absence to nuclear detachment and impaired motility.

By providing validated Nesprin 3 antibody reagents, NSJ Bioreagents supports research into nuclear envelope biology, cytoskeletal dynamics, and disease. Detection of Spectrin repeat containing nuclear envelope protein 3 ensures that researchers can explore how nuclear anchorage and mechanotransduction contribute to cellular function and pathology.

UniProt: Q6ZMZ3

Pathways: Maintenance of Protein Location