DNMT3A antibody

Catalog No. ABIN7880635

The Mouse Monoclonal anti-DNMT3A antibody (Clone 1823CT281-43-36) (ABIN7880635) specifically detects DNMT3A in WB. The antibody is reactive with Human samples.

Quick Overview for DNMT3A antibody (ABIN7880635)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1823CT281-43-36

Product Details anti-DNMT3A Antibody

Purpose: Dnmt3a Antibody

Purification: Protein G purified

Immunogen: Recombinant human Dnmt3a was used as the immunogen for the Dnmt3a antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Dnmt3a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: Dnmt3a

Background: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. [UniProt]

UniProt: Q9Y6K1