Recombinant GGH antibody

Catalog No. ABIN7880852

This Rabbit Monoclonal antibody specifically detects GGH in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant GGH antibody (ABIN7880852)

Target: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GGH antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31G17

Product Details anti-GGH Antibody

Purpose: GGH Antibody / Gamma glutamyl hydrolase

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Gamma glutamyl hydrolase was used as the immunogen for the GGH antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GGH antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the GGH antibody at -20oC.

Target Details for GGH

Target: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Alternative Name: GGH

Background: GGH antibody detects gamma glutamyl hydrolase, an enzyme encoded by the GGH gene. GGH hydrolyzes polyglutamylated folates and antifolate drugs, converting them into monoglutamyl forms that can be transported across membranes. This activity regulates intracellular folate homeostasis and influences the efficacy of antifolate chemotherapeutics such as methotrexate. By controlling folate metabolism, GGH contributes to nucleotide synthesis, DNA replication, and cell proliferation.

GGH antibody is widely applied in oncology, pharmacology, and metabolism research. Elevated GGH expression reduces the retention and potency of antifolate drugs, contributing to chemoresistance in leukemia and solid tumors. Conversely, altered GGH activity affects folate availability and one carbon metabolism, impacting DNA synthesis and repair. By detecting GGH, researchers can study how folate metabolism intersects with cancer biology, drug resistance, and metabolic disease.

Western blot assays detect GGH protein bands in liver and tumor extracts. Immunohistochemistry maps expression in proliferative tissues and tumors, while immunofluorescence highlights cytoplasmic localization in cells actively synthesizing DNA. These approaches provide insight into how GGH distribution correlates with metabolic activity and drug response.

GGH also plays roles in normal physiology, regulating folate supply for rapidly dividing cells in bone marrow, intestinal epithelium, and developing tissues. Dysregulation of GGH has been associated with megaloblastic anemia, cancer progression, and sensitivity to antifolate therapy. By applying GGH antibody, scientists can explore the balance between folate metabolism and proliferation in both health and disease.

GGH antibody from NSJ Bioreagents provides reliable specificity for studying gamma glutamyl hydrolase function. Its strong performance across multiple assays ensures accurate detection of this enzyme in basic research and translational studies of chemotherapy resistance.

UniProt: Q92820

Pathways: SARS-CoV-2 Protein Interactome