ABO antibody

Catalog No. ABIN7881055

The Mouse Monoclonal anti-ABO antibody (Clone A46-B-B10) (ABIN7881055) specifically detects ABO in IF. The antibody is reactive with Human samples.

Quick Overview for ABO antibody (ABIN7881055)

Target: ABO (Histo-blood group A transferase (ABO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABO antibody is un-conjugated

Application: Immunofluorescence (IF)

Grade: Carrier-free

Clone: A46-B-B10

Product Details anti-ABO Antibody

Purpose: ABO Antibody / Blood Group Antigen H Type 2 (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: Human breast cancer MCF-7 cells were used as the immunogen for the ABO antibody.

Isotype: IgM, kappa

Application Details

Application Notes: Optimal dilution of the ABO antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the ABO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for ABO

Target: ABO (Histo-blood group A transferase (ABO))

Alternative Name: ABO

Background: Recognizes the blood group H type 2 antigens, trisaccharide Fuc1 of human origin. The specificity of this MAb has been identified by haemagglutination tests, immunohistochemistry, binding inhibition studies, and absorption experiments performed with synthetic oligosaccharides. The antibody is virtually nonreactive with H type 1 antigen or with closely related type 2 structures (e.g., Y antigen). A46-B/B10 strongly agglutinates human erythrocytes according to the amount of H substance expressed and can, therefore, easily discriminate between blood groups A1 and A2 as well as A1B and A2B (A1 and A1B are not or only weakly agglutinated). In immunohistochemistry, this antibody seems to provide a highly specific reagent for a restricted number of carcinomas and epithelial lineages in tissue sections and in vitro. This was tested on 753 blood samples. It strongly agglutinates human erythrocytes of blood groups 0, A2 and A2B, but not or only very weakly A1 and A1B. This MAb promises to be useful in the determination of A and AB subgroups in blood group serology. This MAb might also be able to define B subgroups. Among 263 B blood samples, 45 (17.1 % ) were negative or only weakly positive with A46-B/B10.

UniProt: P16442