ICAM-3/CD50 antibody

Catalog No. ABIN7881232

The Mouse Monoclonal anti-ICAM-3/CD50 antibody (Clone SPM505) (ABIN7881232) specifically detects ICAM-3/CD50 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for ICAM-3/CD50 antibody (ABIN7881232)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM505

Product Details anti-ICAM-3/CD50 Antibody

Purpose: Anti-ICAM-3 Antibody

Purification: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the anti-ICAM-3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-ICAM-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: ICAM-3

Background: Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.

UniProt: P32942