Recombinant IL12B antibody

Catalog No. ABIN7881269

The Rabbit Monoclonal anti-IL12B antibody (Clone 31I13) (ABIN7881269) specifically detects IL12B in WB, ICC, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for Recombinant IL12B antibody (ABIN7881269)

Target: IL12B (Interleukin 12 beta (IL12B))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 31I13

Product Details anti-IL12B Antibody

Purpose: IL12B Antibody / Interleukin 12 beta / IL12 p40

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human IL12B was used as the immunogen for the IL12B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IL12B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the IL12B antibody at -20oC.

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL12B

Background: IL12B antibody detects interleukin 12 subunit beta, encoded by the IL12B gene. IL12B forms part of two heterodimeric cytokines, interleukin 12 and interleukin 23. In interleukin 12, IL12B pairs with the IL12A subunit to promote Th1 differentiation and interferon gamma production, supporting cellular immunity against intracellular pathogens. In interleukin 23, IL12B pairs with IL23A to stimulate Th17 responses and production of IL17, driving neutrophilic inflammation. Through these dual roles, IL12B is central to both protective immunity and inflammatory disease.

IL12B antibody is widely applied in immunology, inflammation, and translational medicine. By detecting IL12B, researchers can examine how immune signaling shapes host defense and pathology. Elevated IL12B expression contributes to autoimmune diseases such as psoriasis, Crohn disease, and multiple sclerosis. Conversely, defective IL12B leads to increased susceptibility to infection due to impaired Th1 responses.

ELISA quantifies IL12B in serum or culture supernatants. Western blot assays detect IL12B protein bands in activated immune cell lysates. Immunohistochemistry maps expression in lymphoid tissues, while immunofluorescence highlights secretion patterns in dendritic cells and macrophages. These diverse applications make IL12B antibody a versatile tool for immune research.

IL12B has therapeutic relevance as a target of monoclonal antibodies that block interleukin 12 and interleukin 23 signaling. Agents such as ustekinumab neutralize IL12B and provide clinical benefit in psoriasis and inflammatory bowel disease. By applying IL12B antibody, scientists can evaluate pathway activity and therapeutic responses in autoimmune and inflammatory disorders.

IL12B antibody from NSJ Bioreagents delivers strong specificity for analyzing cytokine biology. Its performance across multiple methods supports research into immune signaling and translational medicine.

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation