Recombinant Iba1 antibody

Catalog No. ABIN7881326

The Rabbit Monoclonal anti-Iba1 antibody (Clone 31A74) (ABIN7881326) specifically detects Iba1 in WB, IHC, ICC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Recombinant Iba1 antibody (ABIN7881326)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 31A74

Product Details anti-Iba1 Antibody

Purpose: AIF1 Antibody / IBA1

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Iba1 was used as the immunogen for the AIF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the AIF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the AIF1 antibody at -20oC.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: IBA1 antibody detects allograft inflammatory factor 1 (AIF1), a calcium-binding protein expressed in microglia and macrophages. IBA1 participates in actin cytoskeleton remodeling, enabling immune cells to migrate and phagocytose effectively. In the central nervous system, it serves as a widely used microglial marker, allowing researchers to monitor immune responses within brain tissue. Its expression increases during neuroinflammation, making it valuable for studying disease-related activation states.

Applications of IBA1 antibody include research on Alzheimer's disease, Parkinson's disease, multiple sclerosis, and traumatic brain injury. Microglial activation visualized through IBA1 staining helps define the extent and nature of neuroimmune involvement in these conditions. Beyond neuroscience, AIF1 is also expressed in peripheral macrophages, where it regulates inflammatory pathways and vascular remodeling, linking it to atherosclerosis and transplant rejection models.

Antibodies against IBA1 are validated for immunohistochemistry, immunofluorescence, and western blot. These tools allow precise localization of microglia and provide insights into how immune cells interact with neurons and glia in health and disease. Clone-based products ensure high specificity with minimal background staining.

NSJ Bioreagents provides this IBA1 antibody for research applications in neuroscience, inflammation, and immunology. Alternate names include allograft inflammatory factor 1 antibody, ionized calcium-binding adapter molecule 1 antibody, and microglial marker antibody.

UniProt: P55008

Pathways: Smooth Muscle Cell Migration