Recombinant Keratin Basic antibody

Catalog No. ABIN7881439

The Rabbit Monoclonal anti-Keratin Basic antibody is suitable to detect Keratin Basic in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p), IF and FACS.

Quick Overview for Recombinant Keratin Basic antibody (ABIN7881439)

Target: Keratin Basic

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: KRTH-2147R

Product Details

Purpose: Recombinant Basic Cytokeratin Antibody / Rabbit Monoclonal (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for the recombinant Basic Cytokeratin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Keratin Basic

Alternative Name: Basic Cytokeratin

Background: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

UniProt: Q01546