NG2 antibody

Catalog No. ABIN7881590

The Mouse Monoclonal anti-NG2 antibody (Clone LHM 2) (ABIN7881590) specifically detects NG2 in IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for NG2 antibody (ABIN7881590)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LHM 2

Product Details anti-NG2 Antibody

Purpose: CSPG4 Antibody / NG2

Purification: Protein G affinity chromatography

Immunogen: A 375P cells crude extract was used as the immunogen for this CSPG4 antibody

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the CSPG4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CSPG4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4

Background: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. [UniProt]

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process