MFGE8 antibody

Catalog No. ABIN7881639

This Mouse Monoclonal antibody specifically detects MFGE8 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for MFGE8 antibody (ABIN7881639)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFGE8 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM291

Product Details anti-MFGE8 Antibody

Purpose: MFG-E8 Antibody

Purification: Protein G affinity chromatography

Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the MFG-E8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the MFG-E8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: MFG-E8

Background: Recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interactome