MFGE8 antibody

Catalog No. ABIN7881640

The Rabbit Polyclonal anti-MFGE8 antibody is suitable to detect MFGE8 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for MFGE8 antibody (ABIN7881640)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFGE8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MFGE8 Antibody

Purpose: MFG-E8 Antibody / Lactadherin / Milk Fat Globule

Sequence: FKVAYSLNGH EFDFIHDVNK KHKE

Purification: Antigen affinity purified

Immunogen: Amino acids FKVAYSLNGHEFDFIHDVNKKHKE from the human protein were used as the immunogen for the MFG-E8 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MFG-E8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MFG-E8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: MFG-E8

Background: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interactome