MBP antibody

Catalog No. ABIN7881706

The Rat Monoclonal anti-MBP antibody is suitable to detect MBP in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for MBP antibody (ABIN7881706)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MBP-4273

Product Details anti-MBP Antibody

Purpose: Myelin Basic Protein Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human Myelin Basic Protein was used as the immunogen for this Myelin Basic Protein antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Myelin Basic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein

Background: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4273 recognizes an epitope in the 82-87 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP. MBP/4273 is also used in a 2-site ELISA with clone MBP/4275. Both are used interchangeably as capture and detection antibody.

UniProt: P02686