MYOD1 antibody

Catalog No. ABIN7881721

The Mouse Monoclonal anti-MYOD1 antibody is suitable to detect MYOD1 in samples from Human. It has been validated for IHC (p).

Quick Overview for MYOD1 antibody (ABIN7881721)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MYOD1-7149

Product Details anti-MYOD1 Antibody

Purpose: MyoD1 Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant mouse MyoD1 protein was used as the immunogen for the MyoD1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the MyoD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MyoD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MyoD1

Background: Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. The epitope of this mAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development