MYH11 antibody

Catalog No. ABIN7881733

The Mouse Monoclonal anti-MYH11 antibody is suitable to detect MYH11 in samples from Human and Rat. It has been validated for IHC (p).

Quick Overview for MYH11 antibody (ABIN7881733)

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYH11 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM201

Product Details anti-MYH11 Antibody

Purpose: SM-MHC Antibody

Purification: Protein G affinity chromatography

Immunogen: Human uterus extract was used as the immunogen for the SM-MHC antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Optimal dilution of the SM-MHC antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the SM-MHC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Alternative Name: SM-MHC

Background: Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

UniProt: P35749