Nucleolin antibody (CF®488A)

Catalog No. ABIN7881850

The Mouse Monoclonal anti-Nucleolin antibody is suitable to detect Nucleolin in samples from Human. It has been validated for IF and FACS.

Quick Overview for Nucleolin antibody (CF®488A) (ABIN7881850)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: NCL-902

Product Details anti-Nucleolin Antibody (CF®488A)

Purpose: Nucleolin Antibody

Purification: Protein G affinity chromatography

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Nucleolin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 500 µL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the Nucleolin antibody at 2-8oC, protected from light.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization