Recombinant Nucleolin antibody

Catalog No. ABIN7881852

This Rabbit Monoclonal antibody specifically detects Nucleolin in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant Nucleolin antibody (ABIN7881852)

Target: Nucleolin (NCL)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 30N86

Product Details anti-Nucleolin Antibody

Purpose: NCL Antibody / Nucleolin

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Nucleolin was used as the immunogen for the NCL antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NCL antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the NCL antibody at -20oC.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: NCL

Background: NCL antibody detects nucleolin, a multifunctional nucleolar protein involved in ribosome biogenesis, RNA metabolism, and chromatin organization. Nucleolin is one of the most abundant nucleolar proteins, shuttling between the nucleolus, nucleoplasm, and cytoplasm. Its diverse functions include rRNA transcription, processing, and ribosomal subunit assembly, making it essential for protein synthesis. In addition, nucleolin participates in mRNA stabilization, DNA repair, and chromatin remodeling.

Research using NCL antibody has shown that nucleolin plays crucial roles in cell proliferation and stress responses. Because of its involvement in ribosome production, nucleolin is highly expressed in rapidly dividing cells. In cancer, overexpression of NCL promotes tumor growth, angiogenesis, and resistance to apoptosis. Nucleolin interacts with oncogenic and tumor suppressor pathways, making it both a biomarker and potential therapeutic target.

In addition to cancer, nucleolin is implicated in viral infection, neurodegeneration, and cardiovascular disease. Viruses such as HIV and SARS-CoV-2 exploit nucleolin for entry or replication. In neurobiology, nucleolin regulates synaptic plasticity and neuronal survival under stress conditions. In cardiology, nucleolin modulates stress signaling pathways and contributes to vascular remodeling.

Nucleolin is also a target for therapeutic development. Aptamers, peptides, and small molecules targeting NCL are being explored in oncology and antiviral research. Monitoring NCL expression with antibodies helps evaluate disease progression and therapeutic efficacy.

Antibodies against nucleolin are validated for western blot, immunohistochemistry, immunofluorescence, and ELISA. These reagents provide robust detection across tissues and experimental systems. Clone-based NCL antibodies ensure specificity, supporting reproducible research outcomes.

NSJ Bioreagents provides this NCL antibody for studies in cancer, virology, and cellular stress biology.

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization