PPARA antibody

Catalog No. ABIN7881919

The Mouse Monoclonal anti-PPARA antibody is suitable to detect PPARA in samples from Human and Mouse. It has been validated for WB, FACS, IF and IHC (p).

Quick Overview for PPARA antibody (ABIN7881919)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1331CT894-186-143

Product Details anti-PPARA Antibody

Purpose: PPARA Antibody / PPAR alpha

Purification: Protein G affinity

Immunogen: A recombinant human protein was used as the immunogen for the PPARA antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the PPARA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the PPARA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPARA

Background: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C