Recombinant PNKP antibody

Catalog No. ABIN7882001

The Rabbit Monoclonal anti-PNKP antibody (Clone 30P00) (ABIN7882001) specifically detects PNKP in WB, IHC and IP. The antibody is reactive with Human samples.

Quick Overview for Recombinant PNKP antibody (ABIN7882001)

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PNKP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 30P00

Product Details anti-PNKP Antibody

Purpose: PNKP Antibody / Polynucleotide kinase 3 phosphatase

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PNK / PNKP was used as the immunogen for the PNKP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PNKP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PNKP antibody at -20oC.

Target Details for PNKP

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Alternative Name: PNKP

Background: PNKP antibody detects polynucleotide kinase 3'-phosphatase, a DNA repair enzyme with dual kinase and phosphatase activities. PNKP processes DNA ends by phosphorylating 5' termini and dephosphorylating 3' termini, preparing them for ligation during single-strand and double-strand break repair. This enzyme functions in multiple repair pathways, including base excision repair and non-homologous end joining, ensuring genomic stability after DNA damage.

Research using PNKP antibody has revealed the protein's significance in neurodegenerative diseases and cancer. Mutations in PNKP cause neurological disorders such as microcephaly with seizures and developmental delay (MCSZ), where defective DNA repair leads to progressive neuronal loss. In oncology, PNKP is upregulated in certain tumors, where it contributes to resistance against radiation and chemotherapy by repairing therapy-induced DNA damage. Targeting PNKP is therefore a promising therapeutic strategy to sensitize tumors to genotoxic treatments.

PNKP also plays a role in maintaining mitochondrial DNA integrity, protecting cells from oxidative stress. Its activity is essential for neuronal survival and for preventing age-related genomic instability. Understanding PNKP regulation provides insight into how cells balance repair efficiency with the risk of mutagenesis.

Antibodies against PNKP are validated for use in western blot, immunofluorescence, immunoprecipitation, and immunohistochemistry. These tools allow researchers to track DNA repair activity, analyze protein localization in nuclei and mitochondria, and explore mechanisms of therapy resistance. Clone-based antibodies ensure reproducible results across studies.

NSJ Bioreagents supplies this PNKP antibody to support research in DNA repair, neurobiology, and cancer therapeutics.

UniProt: Q96T60

Pathways: DNA Damage Repair, Nucleotide Phosphorylation