Recombinant PTBP2 antibody

Catalog No. ABIN7882002

This Rabbit Monoclonal antibody specifically detects PTBP2 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant PTBP2 antibody (ABIN7882002)

Target: PTBP2 (Polypyrimidine Tract Binding Protein 2 (PTBP2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PTBP2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31P38

Product Details anti-PTBP2 Antibody

Purpose: PTBP2 Antibody / Polypyrimidine tract binding protein 2

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PTBP2 was used as the immunogen for the PTBP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PTBP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PTBP2 antibody at -20oC.

Target Details for PTBP2

Target: PTBP2 (Polypyrimidine Tract Binding Protein 2 (PTBP2))

Alternative Name: PTBP2

Background: PTBP2 antibody detects polypyrimidine tract binding protein 2, an RNA-binding protein that regulates alternative splicing, mRNA transport, and neuronal differentiation. PTBP2, also known as nPTB, belongs to the heterogeneous nuclear ribonucleoprotein (hnRNP) family and contains four RNA recognition motifs that bind CU-rich elements in pre-mRNA. It is expressed predominantly in neurons and testes, where it orchestrates tissue-specific splicing programs.

Research using PTBP2 antibody demonstrates its central role in neuronal development. During neurogenesis, PTBP2 replaces PTBP1 in post-mitotic neurons, reprogramming splicing to promote synaptic maturation and axonal growth. Knockdown of PTBP2 disrupts neuronal differentiation, leading to defective synapse formation and impaired connectivity. Conditional knockout models confirm PTBP2's requirement for brain development, learning, and memory.

In disease contexts, PTBP2 dysregulation has been linked to neurological and reproductive disorders. Aberrant PTBP2 expression alters splicing of genes essential for synaptic signaling, contributing to autism spectrum disorders, intellectual disability, and epilepsy. In spermatogenesis, PTBP2 regulates transcripts critical for germ cell development, and its loss results in male infertility.

Cancer studies have also implicated PTBP2 in tumor progression. In gliomas and other cancers, PTBP2 expression modulates alternative splicing of oncogenes and tumor suppressors, altering cell proliferation and survival. By influencing splicing choices, PTBP2 may contribute to cancer cell adaptability and therapy resistance.

PTBP2 is also a promising target for therapeutic intervention. Manipulating PTBP2 activity or expression has been explored in reprogramming strategies, where suppression of PTBP2 promotes direct conversion of glial cells into functional neurons. Such approaches have potential applications in regenerative medicine and treatment of neurodegenerative disorders.

Antibodies against PTBP2 are validated for western blot, immunohistochemistry, immunofluorescence, and RNA immunoprecipitation. These reagents enable detection of PTBP2 expression patterns and its association with neuronal transcripts. Clone-based antibodies ensure high specificity across developmental and disease models.

NSJ Bioreagents supplies this PTBP2 antibody for research into RNA regulation, neuronal differentiation, and disease biology.

UniProt: Q9UKA9

Pathways: Ribonucleoprotein Complex Subunit Organization, SARS-CoV-2 Protein Interactome