Recombinant PPM1G antibody

Catalog No. ABIN7882087

The Rabbit Monoclonal anti-PPM1G antibody is suitable to detect PPM1G in samples from Human and Rat. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for Recombinant PPM1G antibody (ABIN7882087)

Target: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PPM1G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 30P06

Product Details anti-PPM1G Antibody

Purpose: PPM1G Antibody / Protein phosphatase Mg2+/Mn2+ dependent 1G

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PPM1G was used as the immunogen for the PPM1G antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PPM1G antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PPM1G antibody at -20oC.

Target Details for PPM1G

Target: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Alternative Name: PPM1G

Background: PPM1G antibody detects protein phosphatase, Mg2+/Mn2+ dependent 1G, a member of the PP2C family of serine/threonine phosphatases. PPM1G is a nuclear-localized enzyme that regulates pre-mRNA splicing, chromatin remodeling, and DNA damage responses. By dephosphorylating specific substrates, PPM1G ensures proper assembly of the spliceosome and contributes to transcriptional control.

Research using PPM1G antibody has demonstrated the enzyme's significance in cell cycle regulation and genome stability. PPM1G participates in the DNA damage response by dephosphorylating gamma-H2AX, a key histone mark of double-strand breaks. This activity supports checkpoint recovery and restoration of normal transcription after DNA repair. In addition, PPM1G regulates the phosphorylation status of spliceosomal proteins, influencing alternative splicing decisions critical for development and stress adaptation.

PPM1G has been studied in cancer, where its overexpression promotes survival of tumor cells by modulating DNA repair and splicing pathways. Aberrant activity may enable oncogenic transcript variants or resistance to DNA-damaging therapies. Conversely, reduced PPM1G function can impair cell survival under stress, underscoring its context-dependent roles in disease. In neurobiology, altered expression of PPM1G has been linked to synaptic signaling and cognitive function, connecting phosphatase activity to brain health.

Antibodies against PPM1G are validated for western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation. These reagents allow researchers to monitor nuclear localization, assess changes in expression, and study interactions with chromatin-associated proteins. Clone-based antibodies provide the specificity needed to distinguish PPM1G from other PP2C family members.

NSJ Bioreagents supplies this PPM1G antibody to support studies in splicing regulation, DNA repair, and cancer biology.

UniProt: O15355