RAB5 antibody

Catalog No. ABIN7882124

The Mouse Monoclonal anti-RAB5 antibody (Clone 3E9-) (ABIN7882124) specifically detects RAB5 in WB, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RAB5 antibody (ABIN7882124)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 3E9-

Product Details anti-RAB5 Antibody

Purpose: RAB5A Antibody

Sequence: KKLPKNEPQN PGANSARGRG VDLTEPTQPT RNQCCSN

Purification: Purified

Immunogen: Amino acids KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN from the human protein were used as the immunogen for the RAB5A antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the RAB5A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RAB5A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: RAB5A

Background: RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity