Recombinant rala antibody

Catalog No. ABIN7882141

The Rabbit Monoclonal anti-rala antibody is suitable to detect rala in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant rala antibody (ABIN7882141)

Target: rala (Ras-related Protein Ral-A (rala))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This rala antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 31R04

Product Details anti-rala Antibody

Purpose: RALA Antibody / Ras-related protein Ral-A

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human RalA was used as the immunogen for the RALA antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RALA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the RALA antibody at -20oC.

Target Details for rala

Target: rala (Ras-related Protein Ral-A (rala))

Alternative Name: RALA

Background: RALA antibody detects Ras-related protein Ral-A, encoded by the RALA gene. Ral-A is a small GTPase belonging to the Ras superfamily that regulates vesicle trafficking, cytoskeletal organization, and signal transduction. Like other GTPases, Ras-related protein Ral-A cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch downstream of Ras signaling. RALA antibody provides a crucial tool for studying cell growth, vesicle exocytosis, and oncogenic pathways where Ral signaling contributes.

Ras-related protein Ral-A functions downstream of Ral guanine nucleotide exchange factors, which activate it in response to Ras and RalGDS signaling. Active Ral-A interacts with effectors such as Sec5 and Exo84, components of the exocyst complex, to regulate polarized exocytosis and vesicle trafficking. Research with RALA antibody has demonstrated that loss of Ral-A disrupts vesicle docking at the plasma membrane, impairing processes such as neurotransmitter release, insulin secretion, and epithelial polarity. This highlights its importance in fundamental cellular logistics.

In cancer biology, Ral-A has been shown to contribute to tumorigenesis independently of Ras. Research using RALA antibody has revealed that Ral-A promotes anchorage-independent growth, invasion, and metastasis in pancreatic and bladder cancers. Unlike Ral-B, which is more closely linked to cell survival, Ral-A is particularly involved in tumor initiation and vesicle trafficking related to oncogenic signaling. Its role as a Ras effector highlights the complexity of Ras-driven oncogenesis and the potential of targeting Ral pathways for therapy.

Beyond cancer, Ras-related protein Ral-A participates in endocytosis, cytoskeletal dynamics, and mitochondrial fission. Studies with RALA antibody have confirmed its localization to the plasma membrane and intracellular vesicles, supporting its roles in trafficking and morphology. Dysregulation of Ral-A has been implicated in metabolic disease and neurological conditions, expanding its significance across multiple fields of study.

RALA antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects Ral-A expression across tissues, immunohistochemistry highlights tissue-specific expression patterns, and immunofluorescence reveals colocalization with vesicle markers and actin structures. Functional assays with RALA antibody enable researchers to link GTPase activation with trafficking, signaling, and cell behavior.

By providing validated RALA antibody reagents, NSJ Bioreagents supports research into small GTPase biology, vesicle dynamics, and oncogenesis. Detection of Ras-related protein Ral-A provides a reliable tool for dissecting how this signaling switch integrates growth and trafficking pathways in both normal and diseased states.

UniProt: P11233

Pathways: Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome