Recombinant RIPK2 antibody

Catalog No. ABIN7882148

The Rabbit Monoclonal anti-RIPK2 antibody (Clone 31R02) (ABIN7882148) specifically detects RIPK2 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant RIPK2 antibody (ABIN7882148)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RIPK2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31R02

Product Details anti-RIPK2 Antibody

Purpose: RIPK2 Antibody / Receptor interacting serine threonine kinase 2

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Receptor interacting serine threonine kinase 2 was used as the immunogen for the RIPK2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RIPK2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the RIPK2 antibody at -20oC.

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background: RIPK2 antibody detects Receptor interacting serine threonine kinase 2, encoded by the RIPK2 gene. This cytoplasmic kinase belongs to the RIP kinase family and is a critical mediator of innate immune signaling downstream of nucleotide binding oligomerization domain (NOD) receptors. By integrating microbial sensing with downstream NF kappa B and MAP kinase activation, RIPK2 helps coordinate proinflammatory gene expression. RIPK2 antibody provides a valuable reagent for studying innate immunity, inflammation, and host pathogen interactions.

Receptor interacting serine threonine kinase 2 is activated by NOD1 and NOD2, intracellular pattern recognition receptors that detect bacterial peptidoglycan. Upon ligand recognition, NOD proteins recruit RIPK2 through caspase activation and recruitment domains, leading to kinase activation and signaling complex formation. Research using RIPK2 antibody has shown that this kinase is indispensable for NF kappa B activation, cytokine production, and antimicrobial defense. By functioning as a central signaling hub, RIPK2 links bacterial detection to adaptive immune responses.

Beyond antimicrobial defense, RIPK2 contributes to autophagy and regulation of cell death. Studies with RIPK2 antibody have demonstrated that it interacts with autophagy proteins to clear intracellular bacteria. Dysregulation of RIPK2 signaling can lead to pathological inflammation, contributing to Crohn disease, Blau syndrome, and other autoinflammatory conditions. Mutations in RIPK2 or its upstream activators alter immune responses and are associated with susceptibility to chronic inflammatory disease.

In oncology, aberrant RIPK2 activation has been linked to tumor progression and immune evasion. Research with RIPK2 antibody has revealed that overexpression or hyperactivation of this kinase promotes survival pathways in cancer cells and contributes to a proinflammatory tumor microenvironment. Inhibition of RIPK2 is being investigated as a therapeutic strategy to reduce chronic inflammation and enhance cancer immunotherapy outcomes.

RIPK2 antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting demonstrates phosphorylation dependent activation, immunohistochemistry highlights expression in inflamed tissues, and immunoprecipitation isolates complexes formed during NOD signaling. These applications make RIPK2 antibody a versatile reagent for both basic and translational immunology.

By supplying validated RIPK2 antibody reagents, NSJ Bioreagents supports research into innate immunity, inflammation, and disease. Detection of Receptor interacting serine threonine kinase 2 provides insight into how innate immune signaling influences health and pathology.

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades