Recombinant RPN1 antibody

Catalog No. ABIN7882169

The Rabbit Monoclonal anti-RPN1 antibody (Clone 32R32) (ABIN7882169) specifically detects RPN1 in WB, IHC, ICC, FACS and IF. The antibody is reactive with Human and Rat samples.

Quick Overview for Recombinant RPN1 antibody (ABIN7882169)

Target: RPN1 (Ribophorin 1 (RPN1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 32R32

Product Details anti-RPN1 Antibody

Purpose: RPN1 Antibody / Ribophorin I

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Ribophorin I was used as the immunogen for the RPN1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RPN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the RPN1 antibody at -20oC.

Target Details for RPN1

Target: RPN1 (Ribophorin 1 (RPN1))

Alternative Name: RPN1

Background: RPN1 antibody detects Ribophorin I, encoded by the RPN1 gene. Ribophorin I is an integral membrane glycoprotein of the rough endoplasmic reticulum and a component of the oligosaccharyltransferase complex. This complex mediates the transfer of core oligosaccharides to nascent polypeptides, a key step in protein N linked glycosylation. RPN1 antibody provides researchers with an important reagent to study protein maturation, endoplasmic reticulum function, and secretory pathway biology.

Protein glycosylation is a critical post translational modification that ensures proper folding, stability, and function of many secreted and membrane proteins. Research using RPN1 antibody has shown that Ribophorin I contributes to substrate recognition and complex stability within the oligosaccharyltransferase machinery. By supporting efficient glycosylation, RPN1 plays a vital role in quality control and trafficking of glycoproteins through the secretory pathway.

Studies with RPN1 antibody have revealed that disruption of Ribophorin I function impairs N linked glycosylation, leading to misfolded proteins and activation of endoplasmic reticulum stress responses. Persistent dysfunction contributes to protein misfolding diseases and disorders of glycoprotein biosynthesis. This makes RPN1 a critical factor in maintaining protein homeostasis.

In cancer biology, RPN1 expression has been linked to tumor progression and therapeutic resistance. Research using RPN1 antibody has demonstrated that overexpression of Ribophorin I supports increased protein synthesis and folding capacity in tumor cells, enabling rapid proliferation and adaptation to stress. Elevated levels of RPN1 have been associated with poor prognosis in certain cancers, positioning it as a potential biomarker.

RPN1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein expression and size, immunohistochemistry localizes RPN1 in rough endoplasmic reticulum rich tissues, and immunofluorescence reveals perinuclear reticular distribution. These applications make RPN1 antibody indispensable in research on protein folding and secretion.

By providing validated RPN1 antibody reagents, NSJ Bioreagents supports studies into glycosylation, endoplasmic reticulum biology, and cancer research. Detection of Ribophorin I allows researchers to investigate how secretory pathway components regulate protein maturation and disease.

UniProt: P04843