RMI2 antibody

Catalog No. ABIN7882182

This Rabbit Polyclonal antibody specifically detects RMI2 in WB, FACS and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RMI2 antibody (ABIN7882182)

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RMI2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RMI2 Antibody

Purpose: RMI2 Antibody / RecQ-mediated genome instability protein 2

Sequence: KMTDLSDNPI HESMWELEVE DLHRNIP

Purification: Antigen affinity purified

Immunogen: Amino acids KMTDLSDNPIHESMWELEVEDLHRNIP from the human protein were used as the immunogen for the RMI2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RMI2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RMI2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RMI2

Target: RMI2 (RMI2, RecQ Mediated Genome Instability 2 (RMI2))

Alternative Name: RMI2

Background: RMI2 is a component of the BLM (RECQL3) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability. This gene is mapped to 16p13.13. RMI1 and RMI2 were present in approximately stoichiometric amounts with other BLM complex components, including topoisomerase-3-alpha (TOP3A), RPA (RPA1), and BLAP250. RMI2 also associated with RMI1 and TOP3A in a second complex. RMI1 and RMI2 interacted directly, and both were essential for stability of the BLM complex. Depletion of either RMI1 or RMI2 depleted the other protein by 80 to 90 % . Chicken DT40 cells depleted of Rmi2 displayed elevated sister chromatid exchange, but other functions of the BLM complex appeared intact. Mutation analysis revealed that interaction between human RMI2 and BLM was essential for suppression of sister chromatid exchange.

UniProt: Q96E14

Pathways: DNA Damage Repair