Secretory Component antibody

Catalog No. ABIN7882222

The Mouse Monoclonal anti-Secretory Component antibody is suitable to detect Secretory Component in samples from Human. It has been validated for IHC (p).

Quick Overview for Secretory Component antibody (ABIN7882222)

Target: Secretory Component

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Secretory Component antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM217

Product Details anti-Secretory Component Antibody

Purpose: Secretory Component Antibody / ECM1

Purification: Protein G affinity chromatography

Immunogen: Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Secretory Component antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Secretory Component

Target: Secretory Component

Background: This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

UniProt: Q16610