SMURF2 antibody

Catalog No. ABIN7882281

This Mouse Monoclonal antibody specifically detects SMURF2 in WB. It exhibits reactivity toward Human.

Quick Overview for SMURF2 antibody (ABIN7882281)

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMURF2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1826CT865-51-28

Product Details anti-SMURF2 Antibody

Purpose: SMURF2 Antibody

Purification: Protein G purified

Immunogen: Recombinant human SMURF2 was used as the immunogen for the SMURF2 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the SMURF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SMURF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SMURF2

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Alternative Name: SMURF2

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. [UniProt]

UniProt: Q9HAU4