Recombinant SNAP25 antibody

Catalog No. ABIN7882412

The Rabbit Monoclonal anti-SNAP25 antibody (Clone 32S42) (ABIN7882412) specifically detects SNAP25 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Recombinant SNAP25 antibody (ABIN7882412)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 32S42

Product Details anti-SNAP25 Antibody

Purpose: SNAP25 Antibody / Synaptosomal-associated protein 25 kDa

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human SNAP25 was used as the immunogen for the SNAP25 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SNAP25 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the SNAP25 antibody at -20oC.

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background: SNAP25 antibody detects synaptosomal-associated protein 25 kDa, a t-SNARE protein critical for synaptic vesicle exocytosis and neurotransmitter release. SNAP25 is anchored to the plasma membrane and forms a ternary SNARE complex with syntaxin and synaptobrevin, which drives vesicle fusion and release of neurotransmitters into the synaptic cleft. This protein is enriched in neurons, especially presynaptic terminals, where it enables rapid and regulated neurotransmission.

Research using SNAP25 antibody highlights its central role in neuronal communication. Knockout mice lacking SNAP25 fail to release neurotransmitters and die at birth, emphasizing its indispensability. Partial loss of function leads to synaptic transmission defects, altered plasticity, and behavioral abnormalities. In humans, mutations in SNAP25 are associated with epilepsy, intellectual disability, and autism spectrum disorders, underscoring its neurological importance.

In disease research, SNAP25 dysregulation has been linked to schizophrenia, ADHD, and neurodegenerative diseases. Altered SNAP25 expression affects synaptic plasticity and cognitive performance. In Parkinson's disease, reduced SNAP25 activity impairs dopamine release, while in Alzheimer's disease, synaptic dysfunction correlates with disrupted SNARE complex assembly.

SNAP25 also plays roles outside the nervous system. In endocrine cells, it regulates hormone exocytosis, including insulin release from pancreatic beta cells. Dysregulation in this context links SNAP25 to diabetes and metabolic disorders.

As a therapeutic target, SNAP25 has been exploited in treatments such as botulinum neurotoxin, which cleaves SNAP25 and blocks neurotransmitter release, producing muscle relaxation. SNAP25 expression levels are also studied as biomarkers of synaptic integrity in aging and disease.

Antibodies against SNAP25 are validated for immunohistochemistry, immunofluorescence, western blot, and ELISA. These reagents provide robust detection of presynaptic proteins, enabling study of neuronal connectivity, disease mechanisms, and therapeutic interventions. Clone-based antibodies ensure specificity across tissues and experimental models.

NSJ Bioreagents provides this SNAP25 antibody for neuroscience research into synaptic vesicle biology, neurological disease, and neuropharmacology.

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport