Recombinant SYVN1 antibody

Catalog No. ABIN7882414

The Rabbit Monoclonal anti-SYVN1 antibody is suitable to detect SYVN1 in samples from Human, Mouse and Rat. It has been validated for WB, FACS, IF, IP and ICC.

Quick Overview for Recombinant SYVN1 antibody (ABIN7882414)

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SYVN1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 32S38

Product Details anti-SYVN1 Antibody

Purpose: SYVN1 Antibody / Synoviolin / HRD1

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human SYVN1 / HRD1 was used as the immunogen for the SYVN1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SYVN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the SYVN1 antibody at -20oC.

Target Details for SYVN1

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Alternative Name: SYVN1

Background: SYVN1 antibody detects Synoviolin, also called HRD1, encoded by the SYVN1 gene. Synoviolin is an endoplasmic reticulum (ER) resident E3 ubiquitin ligase that plays a central role in ER-associated degradation (ERAD), the process that disposes of misfolded proteins from the ER. By tagging substrates with ubiquitin, Synoviolin directs them for proteasomal degradation, thereby maintaining protein homeostasis in the secretory pathway. SYVN1 antibody provides researchers with a critical reagent to study protein quality control, ER stress, and disease.

ER-associated degradation is essential for secretory pathway fidelity. Research using SYVN1 antibody has shown that Synoviolin forms part of the ER membrane-anchored ubiquitin ligase complex, recognizing unfolded substrates and ubiquitinating them for retrotranslocation and proteasomal degradation. This prevents accumulation of misfolded proteins, protecting cells from ER stress and apoptosis. By maintaining proteostasis, SYVN1 safeguards secretory and metabolic tissues.

Aberrant expression of Synoviolin has been linked to cancer, fibrosis, and autoimmune disease. Studies with SYVN1 antibody have revealed that overexpression promotes tumor survival by reducing ER stress-induced apoptosis. In rheumatoid arthritis, Synoviolin overexpression in synovial tissue contributes to pathological proliferation and joint destruction. These findings highlight SYVN1 as both a biomarker and a potential therapeutic target.

Beyond pathology, SYVN1 contributes to normal development and physiology. Research using SYVN1 antibody has shown that knockout mice exhibit embryonic lethality due to defective ERAD, confirming its essential function. In the liver, SYVN1 regulates lipid metabolism and detoxification processes by controlling ER protein load. These functions underscore the importance of Synoviolin in both health and disease.

SYVN1 antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting confirms expression in liver and synovium, immunohistochemistry highlights tissue-specific overexpression in disease, and immunoprecipitation identifies substrates ubiquitinated by Synoviolin. These methods make SYVN1 antibody a versatile tool in cell biology and translational research.

By supplying validated SYVN1 antibody reagents, NSJ Bioreagents supports research into ER quality control, cancer, and immune regulation. Detection of Synoviolin enables researchers to explore how ERAD maintains proteostasis and how its disruption contributes to pathology.

UniProt: Q86TM6

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling