Topoisomerase II alpha antibody

Catalog No. ABIN7882505

The Rabbit Monoclonal anti-Topoisomerase II alpha antibody (Clone FB-20) (ABIN7882505) specifically detects Topoisomerase II alpha in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Topoisomerase II alpha antibody (ABIN7882505)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FB-20

Product Details anti-Topoisomerase II alpha Antibody

Purpose: TOP2A Antibody / Topoisomerase II alpha

Purification: Affinity purified

Immunogen: A synthetic peptide specific to human Topoisomerase II alpha / TOP2A was used as the immunogen for the TOP2A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TOP2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the TOP2A antibody at -20oC.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: The TOP2A gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [RefSeq]

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases