TFAM antibody

Catalog No. ABIN7882519

The Mouse Monoclonal anti-TFAM antibody is suitable to detect TFAM in samples from Human. It has been validated for WB, IHC (p) and FACS.

Quick Overview for TFAM antibody (ABIN7882519)

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 4D9

Product Details anti-TFAM Antibody

Purpose: mtTFA Antibody

Sequence: EMKSWEEQMI EVGRKDLLRR TIKKQRKY

Purification: Affinity purified

Immunogen: Amino acids EMKSWEEQMIEVGRKDLLRRTIKKQRKY were used as the immunogen for the mtTFA antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the mtTFA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the mtTFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: mtTFA

Background: TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

UniProt: Q00059

Pathways: Chromatin Binding