p63 antibody

Catalog No. ABIN7882599

This Rabbit Monoclonal antibody specifically detects p63 in IF, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for p63 antibody (ABIN7882599)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This p63 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: ZR8

Product Details anti-p63 Antibody

Purpose: deltaNp63 Antibody / p40 (azide and preservative free)

Sequence: ENNAQTQFSE PQY

Purification: Protein A affinity chromatography

Immunogen: Amino acids ENNAQTQFSEPQY were used as the immunogen for the deltaNp63 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the deltaNp63 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the deltaNp63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Alternative Name: deltaNp63

Background: P63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) antibody recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.

UniProt: Q9H3D4