Recombinant Vinculin antibody

Catalog No. ABIN7882681

The Mouse Monoclonal anti-Vinculin antibody is suitable to detect Vinculin in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant Vinculin antibody (ABIN7882681)

Target: Vinculin (VCL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RVCL-7287

Product Details anti-Vinculin Antibody

Purpose: Vinculin Antibody

Purification: Protein A/G affinity

Immunogen: A recombinant fragment of human Vinculin (VCL) protein was used as the immunogen for the Vinculin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Vinculin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and Alpha-Actinin. In addition, vinculin, Talin and Alpha-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. Alpha-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2