ICAM-3/CD50 antibody

Catalog No. ABIN7884255

This Mouse Monoclonal antibody specifically detects ICAM-3/CD50 in FACS and WB. It exhibits reactivity toward Human.

Quick Overview for ICAM-3/CD50 antibody (ABIN7884255)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Clone: MA4

Product Details anti-ICAM-3/CD50 Antibody

Purpose: Human ICAM-3 Mouse Monoclonal Antibody (MA4)

Specificity: The antibody recognizes the domains 1-2 of intercellular adhesion molecule (ICAM)-3. This antibody has been shown to block the interaction of apoptotic leukocytes with macrophages/phagocytes.

Purification: Protein G purified

Immunogen: Mammalian recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: ICAM-3

Background:

CD50, Intercellular adhesion molecule 3

Rapid clearing of apoptotic cells by phagocytes may prevent tissue damage. Studies show that ICAM-3 (also known as CD50), expressed on leukocytes, interacts with macrophages during apoptotic cell clearing (Torr et al., 2012). ICAM-3 plays an important role in the recruitment and tethering of macrophages during the process of clearing apoptotic human leukocytes, and ICAM-3 deficient cells have reduced clearance of apoptotic cells by phagocytes. This antibody, (MA4), has been shown to block the interaction of apoptotic leukocytes with macrophages/phagocytes.