Adenosine A3 Receptor antibody (2nd Extracellular Loop)

Catalog No. ABIN7884727

The Rabbit Polyclonal anti-Adenosine A3 Receptor antibody is suitable to detect Adenosine A3 Receptor in samples from Human. It has been validated for WB, FACS and LCI.

Quick Overview for Adenosine A3 Receptor antibody (2nd Extracellular Loop) (ABIN7884727)

Target: Adenosine A3 Receptor (ADORA3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A3 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Adenosine A3 Receptor Antibody

Binding Specificity: 2nd Extracellular Loop, AA 152-166

Purpose: A rabbit polyclonal antibody to Human Adenosine A3 Receptor (extracellular)

Predicted Reactivity: Human only

Purification: Affinity purified on immobilized antigen.

Immunogen: Peptide KLTSEYHRNVTFLSC, corresponding to amino acid residues 152 - 166 of human ADORA3

Isotype: IgG

Application Details

Application Notes: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Adenosine A3 Receptor

Target: Adenosine A3 Receptor (ADORA3)

Alternative Name: ADORA3

Background:

Synonyms: Adenosine receptor A3, A3AR, AA3R, ADORA3

Description:

Adenosine A3 receptor (also known as ADORA3), is a member of the G protein-coupled receptor (GPCR) family that plays a crucial role in regulating a wide range of physiological processes, especially those related to inflammation, immunity, and cell survival. It is one of four receptors activated by adenosine, a purine nucleoside that functions as a local modulator of cellular activity in response to stress, hypoxia, or tissue damage.1

Encoded by the ADORA3 gene, this receptor is primarily coupled to Gi/o proteins, resulting in the inhibition of adenylate cyclase and a subsequent decrease in cyclic AMP (cAMP) levels. Beyond this classical signaling pathway, ADORA3 activation triggers multiple intracellular cascades, such as MAPK/ERK, PI3K/Akt, and PLCβ/DAG/IP3, which together regulate critical cellular functions including apoptosis, proliferation, cytokine production, and oxidative stress responses.1,2

The ADORA3 receptor is broadly expressed across various tissues, including immune cells, the central nervous system, cardiac tissue, lungs, and liver. In immune cells such as neutrophils, mast cells, and lymphocytes, ADORA3 plays a key anti-inflammatory role by modulating cytokine secretion and reducing the activation of pro-inflammatory pathways. This has made it a target of interest in the treatment of autoimmune and inflammatory diseases like rheumatoid arthritis, inflammatory bowel disease, and psoriasis.2,3,4

In oncology, ADORA3 is often overexpressed in various tumors, including breast, colon, and melanoma cancers. Its activation in cancer cells can lead to apoptosis and inhibition of angiogenesis, positioning it as a potential target for anticancer therapies. Interestingly, it may exert opposite effects in different cellular environments, reflecting its complex and context-dependent nature.4,5

In summary, the ADORA3 receptor is a multifaceted regulatory molecule at the crossroads of inflammation, immunity, cardiovascular protection, and cancer biology. Its unique signaling capabilities and tissue-specific roles make it a compelling target for drug development, particularly in diseases characterized by chronic inflammation or uncontrolled cell growth.

Gene ID: 140

UniProt: P0DMS8

Pathways: Hormone Transport, cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process